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| Cell line: | GH |
| DSMZ no.: | ACC 811 |
| Species: | human (Homo sapiens) |
| Cell type: | testicular germ cell tumor |
| Origin: | established in 1980 from a human testicular teratocarcinoma of a male patient of unknown age; cells are described in the literature to express mRNA for endogenous retrovirus and virus-like particles; they are further described in the literature to be ultrasensitive to cisplatin |
| Reference(s): | 18139, 18140, 18141, 15195 |
| Biosafety level: | 2 |
| Risk assessment: | As described in literature, the GH cells produce human teratocarcinoma derived viruses (HDTV) which are identical to human endogenous retrovirus K (HERV-K). HERV-K is assigned to biosafety level 2 in Germany. The cell culture supernatant shows a marginal reverse transcriptase activity in the cell culture supernatant which can slightly be stimulated by 5-azacytidine and strongly applying 5-iodo-2-deoxyuridine. The activity was determined employing the product enhanced reverse transcriptase assay (PERT) |
| Permissions and restrictions: | A, B |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | polygonal cells growing adherently in monolayers and discrete colonies |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:2 - 1:4 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | cell harvest of ca. 8 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV-, HERV-K + |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
