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Cell line: | JK-6 |
DSMZ no.: | ACC 860 |
Species: | human (Homo sapiens) |
Cell type: | multiple myeloma |
Origin: | established from peripheral blood of a 75-year-old male with multiple myeloma in 1992. Cells were originally IL-6 dependent but became independent during the further course of establishment. Described to carry mutations in the K- and N-RAS genes. More cytogenetic and immunological data, including HLA types are available upon request to mutz@dsmz.de |
Reference(s): | 18188 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single round (plasmablasts-like) cells growing in suspension |
Medium: | 80% RPMI 1640 + 20% FBS + 2 mM L-glutamine |
Subculture: | seed out at about 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 48 hours |
Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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