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| Cell line: | COR-L23 |
| DSMZ no.: | ACC 882 |
| Species: | human (Homo sapiens) |
| Cell type: | large cell lung carcinoma |
| Origin: | established in 1985 from a pleural effusion
of a 62-year-old male with a giant cell anaplastic carcinoma of the lung; described to have a c-myc amplification, a kras mutation and to form tumors in nude mice. Alias name: COR-L23P (P=parental); cells are sensitive towards doxorubicin and a multidrug-resistant subclone is available (COR-L23R, ACC 883); listed in the CCLE (ref 18232) |
| Reference(s): | 18225, 18226, 18227, 18228, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid, very large, often multinucleate cells growing adherently as monolayer; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 1 x 106 cells in a 175 cm2 flask; split semi-confluent 1:5 - 1:10 twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 10-12 x 106 cells/175 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
