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Cell line: | 8-MG-BA |
DSMZ no.: | ACC 432 |
Species: | human (Homo sapiens) |
Cell type: | glioblastoma |
Origin: | established from the frontal brain lobe of a 54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment) |
Reference(s): | 14846 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like, adherent cells growing as monolayer |
Medium: | 80-85% MEM (with Earle's salts) + 15-20% h.i. FBS |
Subculture: | split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2; optimal density at ca. 5-10 x 106/175 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 100 hours |
Harvest: | cell harvest of maximal 15 x 106/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X, +1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36), der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14), i(7p), i(8q), add(8)(p21)x1-2, del(9)(q32q34)/add(9)(q32), add(11)(?q13), del(12)(q14), del(18)(q22.2), add(19)(p13.1) - sideline with del(3)(p11), der(6;9)(p10;q10) - 2-4 acf present in 31% cells |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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