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Cell line: | MUG-Lucifer-prim |
DSMZ no.: | ACC 958 |
Species: | human (Homo sapiens) |
Cell type: | clear cell sarcoma |
Origin: | established in 2019 from the primary tumor of a 23-year-old woman with clear cell sarcoma of the knee joint; sister cell line of the metastasic tumor is also available (MUG-Lucifer-met ACC 959) |
Reference(s): | 40814 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small, round, polygonal and spindle-shaped cells growing in suspension and adherently; semi-adherent, about 50-80% of cells are in suspension, 10-20% are loosely adherent, 10-20% are adherent; image; image; image |
Medium: | 90% DMEM/F12 with Glutamax + 10% h.i. FBS |
Subculture: | seed out at 0.4-0.8 x 105 cells/cm2; split culture 1:6-1:10 every 5 days; it is important to collect all (suspended and adherent) cells, use trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | about 30-40 hours |
Harvest: | 0.3-0.6 x 106 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MUG-Lucifer-met |
Molec. Genetics: | fusion gene EWSR1::ATF1 was verified by RT-PCR;
Reported whole or partial chromosome gains of chromosomes 5, 7, 8, and 17, and partial losses of chromosomes 9 and 15 were confirmed by SNP-array analysis. In contrast to MUG-Lucifer-met, no partial gain of chromosome 1 was detected. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV -1/2 -, XMLV -, |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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