| | |
|---|
| Cell line: | CL-52b |
| DSMZ no.: | ACC 843 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatocellular carcinoma |
| Origin: | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat. Described to exhibit chromosomal instability and to induce cholangiohepatocellular carcinomas in nude mice |
| Reference(s): | 14658, 18172 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells, some with spindle-like extensions growing adherently in monolayers and clusters |
| Medium: | 80-90% MEM (with Earle's salts) + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/175 cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | cell harvest of 10-12 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | n.a. |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Viruses: | PCR: MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
