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| Cell line: | HCC-366BL |
| DSMZ no.: | ACC 895 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells |
| Origin: | lymphoblastoid cell line established from peripheral blood of a 80-year old femal with non-small lung carcinoma; autologous carcinoma cell line HCC-366 is available (DSMZ ACC 492) |
| Reference(s): | 15046, 14567, 14849, 14821 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and partly in aggregates in suspension; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 0.5 x 106 cells/ml; maintain at 0.2-2 x 106 cells/ml; split 1:10 to 1:20 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 15-24 hours |
| Harvest: | maximal density of 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, CD138 -, HLA-DR +
; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the autologous cell line HCC-366 |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
