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| Cell line: | MCF-7GFP |
| DSMZ no.: | ACC 983 |
| Species: | human (Homo sapiens) |
| Cell type: | breast adenocarcinoma |
| Origin: | established in 2009 from the original MCF-7 by transduction with lentiviral SIN vector expressing constitutively eGFP.
(MCF-7 was established in 1970 from the pleural effusion of a 69-year-old woman with metastatic mammary carcinoma after radio- and hormone therapy; available at DSMZ, ACC 115) |
| Reference(s): | 40821, 40872 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | MCF-7 cells were transduced with the lentiviral SIN vector pRRL.PPT.SF.GFP.pre#. The transduction method is an approved third generation safety measure to prevent the formation of replication competent retroviruses. According to German legislation, the genetically modified cells are assigned to safety group S1. |
| Permissions and restrictions: | A;D |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing as monolayers;
bright green fluorescence in nucleus and cytoplasm (almost 100% GFP positive cells by Flow Cytometry); image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 1x non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin |
| Subculture: | seed out at ca. 0.5 x 106 cells in a T25 flask; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-24 hours |
| Harvest: | about 20-25 x 106 cells in a T75 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Plasmocin
thereafter negative in PCR assays |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the unmodified MCF-7 |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
