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Cell line: | OMEGA-E |
DSMZ no.: | ACC 253 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line |
Origin: | derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by "codon wobbling"; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) |
Reference(s): | 14783 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | cells grow as adherent fibroblastic monolayer; image; image; image; image |
Medium: | 90% DMEM + 10% NCS |
Subculture: | seed out in a T75 flask and split confluent culture about 1:5 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 20-30 hours |
Harvest: | cell harvest of about 5-8 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypertriploid karyotype - 71(63-73)<3n> - 1-2 dm (24% cells) |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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