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| Cell line: | R1 |
| DSMZ no.: | ACC 56 |
| Species: | fish - Chinook salmon (Oncorhynchus tshawytscha) |
| Cell type: | liver |
| Origin: | established from the liver of a Chinook salmon (Oncorhynchus tshawytscha) by spontaneous immortalization. Cell line is used in toxicity assays and waste water control (to reduce fish tests) |
| Reference(s): | 14350, 18247 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, fibroblastoid cells growing as monolayerimage; image
image |
| Medium: | 90% Medium 199 (25 mM HEPES) with Earle's salts with L-glutamine + 10% h.i. FBS |
| Subculture: | cell growth is extremely slow; split confluent culture 1:2 every 7 days using TNE (50 mM Tris, 0.1 M NaCl, 5 mM EDTA, pH 7.5) at +4 °C for 10 min; culture at room temperature (about 20 °C) without CO2 (keep lid of flask closed); cells can be stored temporarily (up to 1 week) at 4 °C; seed out at ca. 0.1 x 106 cells/ml |
| Incubation: | at room temperature (about 20 °C) without CO2 |
| Doubling time: | about 160 hours |
| Harvest: | about 5 x 106 cells/175 cm2 - saturation density of ca. 10 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated by the depositor with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus tshawytscha |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
