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Cell line: | YK-M2 |
DSMZ no.: | ACC 949 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established in 1985 from the peripheral blood of a 31-year-old man with acute monoblastic leukemia pretreated with chemotherapy and irradiation. Cells are described as near triploid, expressing Fc-gamma receptors and differentiation could be induced by vitamine D |
Reference(s): | 40736 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | mostly round or oval cells growing singly in suspension; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at about 1 x 106 cells/ml; maintain at 0.8 - 1.5 x 106 cells/ml and split carefully (approx. 1:2 every 2-3 days) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 50 hours |
Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR +, cy IgG+, cy IgM -, cy kappa (+), cy Iambda -, cy CD3 -, cy CD68 +, cy CD79a -;
image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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